tissue monkey cards 2

tissue monkey cards 2

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allo bone age

12 - 65/55

allo skin age

12 - 85

JR age

12 - 35

RTI bone age

15 - 60

RTI skin age

15 - 85

pericardium age

15 - 85

heart age

0 - 50

SV age

15 - 55 (♂ only)

AI age

15 - 45

DTA age

15 -34

nerve age

12 - 60

lifecell age

14

allo bone gloves

16

allo skin fluids

2 allo RPMI, 2 alcohol

donor prep

✓ ID, exam, blood, wash, wet shave, wash, towel, alcohol

allo skin prep

CHG scrub 5/2.5²

arch vessel length

2 cm

PA length

3 cm

aorta past LSC length

2 cm

DTA length

10 cm

AI aorta length

10 cm

AI iliac length

10 cm

SV minimum length

60 cm

SV branches length

2 cm

AI branches length

3 cm

RTI skin minimum

18 x 21 cm (post shrinkage) or multiples thereof

tendon length minimum

25 cm

JR technique

tendons come off, include naviculars

RTI leg en-bloc technique

tendons stay on, exclude naviculars

heart rinse

∆ gloves, 2 L NS

SV rinse

∆ gloves, 500 cc NS

AI rinse

∆ gloves, 2 L NS

heart package

liter of saline, double bag and zip, bucket

AI package

liter of saline, double bag and zip, bucket

vein package

jar, single green bag

RTI bone package

(laps), bag, zip, label.
sidebag, zip, label, bag, zip.
totalbag, zip.

allo bone package

culture, laps, bag, zip, label, bag, zip

JR package

MEM laps, extra outer bag

allo skin package

skin - double bag
cultures - parafilm, biohazard bag

RTI soft tissue package

three bags, two labels.
The 2nd and 3rd bags can be collective (eg, for fascia).

nerve package

each quadrant and culture double bagged, then cultures collectively bagged

cryolife ship

box, bag, styrofoam, bag, bucket, ice, zip, blood (vein), diaper, lid, zip, paperwork, tape box

RTI ship

box, cooler, red bag, ice, tissue, ice, zip, paperwork/lung biopsies/blood, tape cooler, tape box

RTI bone gloves

8

vascular gloves

4

RTI skin gloves

5

allo skin gloves

10

pericardium gloves

2

lifecell skin gloves

5

allo skin ship

skin in ziploc, cultures in ziploc, two double ziploc icebags, tape lid, paperwork, tape box

vein solution

DMEM-papaverine 2cc in 500

nerve ship

box, bag, styrofoam, diaper, bag, quadrants, ice, zip, cultures, lid, zip, paperwork, tape box

lifecell skin fluids

1 lifecell RPMI, 2 alcohol

lifecell skin culture technique

1 cm² x 3, all in one tube, miniziploc

lifecell skin package

double bag with a bottle of RPMI
cultures- miniziploc and zip to the skin bag

RTI lung biopsies ship

formalin, parafilm, biohazard bag, collective bag, next to redbag

RTI blood ship

cylinder, biohazard bag, next to red bag (in the cooler)

full case cryolife ship rule

AI goes alone

allo bone immediate ship

box, styrofoam, diapers, white bag, ice, tissue, ice, zip, tape lid, paperwork, tape box

adipose age

12 - 65/55

immediate allo ship age

40

allo bone label

donor number, date, initials

RTI bone label

donor number, checkmarks

RTI skin label

donor number, skin, posterior torso/right thigh/left thigh

nerve label

donor number

pericardium label

donor number, pericardium

cryolife ship

box, bag, styrofoam, bag, bucket, ice, zip, blood (vein), diaper, lid, zip, paperwork, tape box

allo skin label

donor number, skin, anterior/posterior, (x/x)
culture: donor number, culture, anterior/posterior, (x/x)

lifecell skin label

skin: donor number on inner bag
cultures: skin culture, donor number on little tube

heart label

donor number, heart, date, initials, on side of bucket and lid

AI label

donor number, aortoiliac, date, initials, on side of bucket and lid

SV label

donor number, saphenous vein R/L, date, initials, on side of jar and lid

JR label

donor number, checkmarks

JR gloves

12

bone only gloves

12

adipose solution setup

hyclone/dmem 5/500
dmem/cx 50/50

adipose package

adipose + 2 hyclone/DMEM in jar (1:1).
50 cc plain DMEM + 50 cc adipose in each culture.
double bag jar and each culture

adipose/ allo bone ship

box, styrofoam, diaper, bag, ice, tissue bag, adipose jar, cultures, ice, zip, lid, paperwork, tape box

adipose label

donor number, date, adipose.
cultures: donor number, date, "adipose culture".

experimental freehand skin prep

CHG scrub 5/2.5²
alcohol rinse
pat dry
CHG paint


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